A study using coupled X-ray imaging and diffraction to characterize the biomechanics of a replica root-soil system.
Authors

James Le Houx

Daniel McKay Fletcher

Alberto Leonardi

Katherine A. Williams

Nancy Walker

Fernando Alvarez-Borges

Ebrahim Afsar Dizaj

Madhu Murthy

Ronan Smith

Liam Perera

Navid Aslani

Andrew James

Sharif Ahmed

Tiina Roose

Siul Ruiz

Published

July 3, 2025

Publication Dataset

Abstract

Soil compaction and escalating global drought increase soil strength and stiffness. It remains unclear which plant root biomechanical mechanisms/traits enable growth in these harsh conditions. Here, we combine synchrotron X-ray computed tomography with spatially resolved X-ray diffraction to characterize the biomechanics of a replica root-soil system. We map the strain field around the root tip analog, finding strong agreement with finite element simulations, thereby demonstrating a promising new in vivo measurement protocol.

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